Clostridium didicile antigen rai sai kuaj kab

• Yooj yim siv
• Tsis muaj cov cuab yeej siv
• High rhiab heev thiab muaj qhov tshwj xeeb
• Cov txiaj ntsig sai ntawm 10 feeb

Tus kws tshaj lij (cov tswv cuab ntawm cov neeg genus clostridium) yog anaerobic, cov kab mob motile, ubiquitous nyob rau hauv qhov, thiab tshwj xeeb tshaj yog muaj kev sib cav hauv av. Nyob rau hauv lub tshuab tsom, lawv tshwm sim ntev, tsis xwm yeem nruas (feem ntau cov nruas- los yog ntxooj-puab) cov hlwb nrog kev zom ntawm lawv lub davhlau ya nyob twg. Nyob rau hauv gram staining, clostridium difficile (c. Difficile) hlwb yog gram-zoo thiab qhia zoo kev loj hlob ntawm
Ntshav agar ntawm tib neeg lub cev kub nyob rau hauv qhov tsis muaj pa oxygen. Thaum cov kab mob tau tsim cov hom dej txi uas muaj peev xwm tiv taus cov kab mob huab heev uas nquag cov kab mob tsis tuaj yeem ua siab ntev. Closast tus kab mob strawber yog txuam nrog kev kho mob dav hlau thiab yog qhov ua rau mob plab zom mov hauv tsev kho mob. Kab mob ntawm C. Difficile tsim ob cov protein exotoxins, toxin a thiab toxin b, uas ua rau mob colonic mucosal raug mob thiab mob. C.Difficile yuav raug tsim tsa hauv tib neeg txoj hnyuv; 1,2,3,3it yog tam sim no nyob rau hauv 2-5% ntawm cov neeg laus.

Tso cai rau kev sim, hnoos qeev, tsis ncav cuag chav sov (15-30 degree) ua ntej xeem.
1. Txhawm rau kom sau cov quav fecal:
Sau cov quav txaus ntawm cov quav (1-2 ml lossis 1-2 g) Cov txiaj ntsig zoo tshaj plaws yuav tau txais yog tias qhov pom tau ua hauv 6 teev tom qab sau. Cov ntawv sau cia yuav muab khaws cia rau 3 hnub ntawm 2-8 degree yog tias tsis kuaj hauv 6 teev. Rau lub sij hawm ntev cia, cov quav muag yuav tsum tau ceev cia hauv qab -20 degree.
2. Rau cov txheej txheem quav fecal:
Rau cov khoom siv khov kho:
Unscrew lub hau ntawm cov quav me sau rau cov quav rau hauv cov quav rau hauv tsawg kawg yog 3 mg ntawm cov quav (sib npaug rau 1/4 ntawm pea). Tsis txhob daus cov quav.
Rau cov hnoos qeev:
Unscrew lub hau ntawm qhov quav me sau raj, tuav cov quav quav, thiab tom qab ntawd ua kua roj (kwv yees li 80 μl) mus rau hauv lub patchcen sau lub raj nrog extraction buffer. Zawm lub CAP rau hauv lub raj ntim sau cia, thiab tom qab ntawd co lub raj ntim khoom sib xyaw kom sib xyaw thiab rho tawm tsis. Tawm ntawm lub raj nyob ib leeg rau 2minnutes.

3. Nqa hnab rau chav tsev kub ua ntej qhib nws. Tshem tawm cov kab mob xeem los ntawm cov ntawv ci xaub thiab siv nws sai li sai tau. Cov txiaj ntsig zoo tshaj plaws yuav tau txais yog qhov kev xeem ua tau tom qab qhib cov ntawv ci nyiaj.
4. Ntsuas cov hnoos qeev ntawm lub raj ntseg thiab qhib lub hau rau ntawm lub raj ntim khoom. Invert cov quav Zam kev ua npuas ua npuas rau hauv cov hnoos qeev. Saib daim duab hauv qab no.
5. Nyeem cov txiaj ntsig ntawm 10Minutes. Tsis txhob nyeem cov txiaj ntsig tom qab 20 feeb

Cim npe nrov: Clostridium didicile antigile antigile kev kuaj hniav sai sai, Tuam Tshoj Clostridium didicile antigile antigile antigen kev ntsuas nrawm nrawm nrawm